Fort Nassau was established in 1627 as a Dutch trading post. The remains of the fort is located approximately 88 kilometres (55 miles), from the mouth of the Berbice River (right bank) and borders Torani Creek. The fort was first managed privately by the Van Pere family, led by Abraham Van Pere, a director of the Zeeland Chamber of the West Indian Company (WIC) or Geoctroyeerde Westindische Compagnie (WIC) which was founded in 1621. The first location of the fort is believed to be near the Wiruni River and it was described as a small timber enclosure surrounded by a small cannon and palisades.
From 1629-1630, the fort was moved 80 kilometres (49.71 miles) from the mouth of the Berbice River. It was rehabilitated in 1678 and in 1684, enlarged by Cammandeur Lucas Courdie. By 1720, the Van Pere family lost control of the fort and the Society of Berbice took command of all its assets. In 1733, the fort was ordered to be enlarged by Governor Bernard Waterham. On February 23, 1763, the 1763 Rebellion had erupted on plantation Magdelenenburg along the Upper Canje River. By March 08, the uprising had reached the fort and Commander Wolfort Simon van Hoogenheim along with the other colonizers was forced to flee; everything except the Lutheran church was destroyed.